This expression pattern was also confirmed by direct detection of. Microcystinlr promotes zebrafish danio rerio oocyte in vivo. Results loss of mir145 leads to defects in heart and gut development. In the present study, we used the zebrafish ovarian follicle as a model to. Given that several gut functions and immune genes are conserved between zebrafish and mammals, the zebrafish is an interesting model organism to investigate fundamental processes underlying intestinal inflammation and injury cheesman and guillemin, 2007, yang et al. The rapid specification and differentiation of the embryonic zebrafish gut is essential to provide contractility for the digestion of food. The role of micrornas in modulating gut epithelial or smooth muscle differentiation is currently not known. In early zebrafish development between 16 and 24 hpf, mir145 is expressed ubiquitously at very low levels fig. One of the early choices in the differentiation of epithelial cells is the distinction of whether a cell will become an enterocyte or a secretory cell fig. Lh signaling is required for oocyte maturation in fish and other. In early zebrafish development between 16 and 24 hpf, mir145 is.
S1a with increasingly specific expression in the heart, ear, and pharyngeal arches between 24 and 72 hpf. Silver nanoparticles induce oocyte maturation in zebrafish danio. A new zebrafish model of orointestinal pathogen colonization. Interestingly, preliminary studies with a zebrafish ortholog of the atonal1 gene unpublished, which in mammals directs differentiation of goblet, enteroendocrine and paneth cell intestinal lineages from a multipotent progenitor yang et al. Intestinal growth and differentiation in zebrafish. Thus, the adaptive and innate branches of the immune system are remarkably conserved across jawed fishes teleosts and. The zebrafish intestine is a simple tapered tube that is folded into three. Here we developed a new experimental approach for direct analysis. Similarly, alkaline phosphatase activity is an indicator of gut epithelial maturation and normally appears in the zebrafish intestinal lumen by 72 hpf. Loss of mir145 leads to defects in heart and gut development. Although we cant provide direct evidence to approve the quality of. Igfs mediate the action of lh on oocyte maturation in zebrafish. For gene knockout studies, ab strain zebrafish danio rerio were. Proceedings of the national academy of sciences of the united.
Proximal intestinal diversion at segment 1 s1, equivalent to jejunum was performed in adult male zebrafish. Here we developed a protocol to raise microbefree zebrafish larvae fed on microbefree live food. Pdf mir145 directs intestinal maturation in zebrafish. Microcystinlr promotes zebrafish danio rerio oocyte in vivo maturation by activating erk12mpf signaling pathways, and camp is.
Morphological and molecular evidence for functional organization. We show that alkaline phosphatase activity is completely absent in mir145 morphants at 96 hpf, also indicating a lack of functional maturation fig. Adaptive immune maturation in zebrafish is an active research topic within the. The zebrafish as a model to study intestinal inflammation.
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